오성바이오

Amisan-1000(Brand new/Innovative Booster)

"Amisan-1000" is including the specialized & Innovated Immunity substance, in order to reinforce the Immunity in the Livestock.

As an auxiliary feed of optimizing amino acid composition, Feed Palatability is increased, the Digestion Rate is improved, and the Growth Rate is improved.

Amisan-1000 is a liquid supplementary feed produced through technology of optimizing essential amino acids composition that we have learned by a research and development for a long time, and this product is produced with herbal medicines ingredient that we have gained through know-how from field experience for many years.

As this product used of L-lysine, an essential amino acid, as main ingredient, so disease condition of a sick pig and an atrophied pig is improved rapidly through immunity enhancement in case of feeding for livestock.

In addition, by decreasing stress and increasing feed palatability, we can expect heath improvement effects as an increase of appetite, and a death rate decreases because this product prevents diseases from virus and bacteria.
Also, by decreasing side effects of vaccines and antibiotics, we can anticipate environmentally friendly livestock farming management.

Recently the Importance of supplementary feed has been highlighted as time goes by, as the growing reluctance of customers for pork used antibiotics.
In modern livestock farming, the raising large number of livestock in intensive conditions has led to the use of antibiotics for treatment and prevention of diseases.

However, due to the fact that antibiotics can harm humans who consume meat for various reasons in spite of great effect of antibiotics, as a result, domestically regulations on antibiotics have been tightened, and therefore antibiotics are not being used in the mixing feed currently.

For this reason, because antibiotics are not used, many diseases break such as PRRS, respiratory diseases etc, and we are making great efforts to develop antibiotic substitute.
As this supplementary feed of our company is to improve health and immunity of livestock, and to be helpful for productivity increasing, it is being introduced to modern livestock farms as a new feed additive.

Efficacies and Effects

- Rapid recovery of a sick pig or an atrophied pig on account of immunity improvement.

- Strengthening Immunity and relieving stress due to the supply of essential amino acids.

- Improving growth rate in case of feeding this supplementary feed together with water to a weaning piglet due to proper combination of essential amino acids.

- Decreasing death rate by less than 4 % to 5 % in case of raising from weaning piglets to 70-day-old pigs.

- Reducing mortality rate by less than 2 % to 3 % in case of raising from 70-day-old pigs to raising pigs till shipment day.

- Growth normally through decreasing a rate of occurrence of PMWS disease in case of raising from weaning piglets to swine of 30kg weight.

- Activation of metabolism in the body due to increase of intestinal effective microorganisms.

- facilitating return of estrus in sows after weaning, and increasing suckling piglets weight due to milk of pig increase of sows that farrowed.

- Reducing digestive diseases due to improving digestive absorption rate of weaning piglets.

- Strengthening resistance to disease and decreasing mortality rate due to prevent of bacterial and virus invasion.

- Faster shipping schedule of non-fat pigs and greater feed efficiency.

Productivity of Amisan-1000

- Maximizing feed additive effect due to watering together with this product.

- It is easy to protect the original technology and to enlarge the supply of raw materials, because of being made by domestic technology.

- It is easy to purchase again on account of added value increasing due to the effect of productivity improvement of livestock farms.

- Environmentally friendly livestock farming management is possible as result of the effect reducing overdose of vaccines and antibiotics.

- This company was picked up to beneficiary of main industrial enterprise support service (non-R&D) in Chung Nam region in 2017 and with taking the support of Soon Cheon Hyang University, the effect and efficacy of this product was proved through the specifications test being done by a certified organization.

I. Effects of the supply of Amisan-1000 on the growth rate, feed intake volume, FCR (Feed Conversion Rate) in weaning piglet

When a test was started, 21-day-old 40 weaned piglets that were a ternary hybrid [Land-race x Yorkshire x Duroc] of the weight of 6.28kg ~6.86kg was selected randomly and it was done a field test for 30 days. The field test was conducted at a commercial swine farm of 10,000 sows in Gochang Gun region.

It consisted of 20 pigs per group, and it was grouped into test group and control group to be divided room.
A used feed is a feed of weaning piglet made by P company (a new born piglet feed 1 step), and both test group and control group piglets had eaten that feed freely.
Test group was watering by diluting 1L of Amisan-1000 together with 1 ton of water, and control group was watering normal water freely.

Table 1. Effects of Amisan-1000 on growth rate, feed intake, feed conversion rate in weaning piglet

Items	Control group	Amisan-1000 group
Initial BW (g) Final BW (g) ADG (g) DFI (g) FCR	6.59 16.75 362 586 1.62	6.48 17.98 410 632 1.54

 

Table 2. Effects of Amisan-1000 feeding on blood chemical properties in weaning piglet

Items	Control group	Amisan-1000 group
WBC, 103/ul RBC, 106/ul HGB, g/dl HCT, % MCV, fl MCH, pg MCHC, dl PLT, 103/ul IgG, mg/dl	21.48 6.68 9.85 30.75 46.23 14.61 32.48 328.2 225	24.83 6.97 10.58 32.49 47.45 15.17 32.88 425.5 284.6

Average Daily weight Gain (ADG) increased in Amisan-1000 group (410g) compared to control group (362g). In case of Daily Feed Intake (DFI), Amisan-1000 group (632g) increased a little bit in comparison to control group (586g), but Feed Conversion Rate (FCR) increased greatly as a result in Amisan-1000 group (1.62) compared to control group (1.54).

Feed intake increasing was caused by immunity improvement, feed conversion rate improvement was caused from increase of digestive absorption rate by functional, physiological active substance in Amisan-1000.
In the result of Blood Properties Analysis (Complete Blood Count) & Ig G Content Analysis, Amisan-1000 group increased Leukocyte (White Blood Cell) and Ig G, and concentration of Erythrocyte (Red Blood Cell) increased, so overall blood circulation improvement effect appeared.
Increasing Ig G, an immunoglobulin, indicated activation of cells responsible for humoral immunity, suggesting that Amisan-1000 increases antiviral resistance against viruses. This may solve the problem of PRRS disease which is a problem in young weaning piglets.

II. Increasing effect of pregnancy rate in sows

The following table is a table of pregnancy rates of a livestock farm that has been suffering from continuous pregnancy rate decrease since 2016. This is the case that a pregnancy rate increased after 30 days from starting feeding of Amisan-1000 in October 2018.

Table 3. Increase in pregnancy rates after feeding of Amisan-1000 (January 2016 ~ December 2018)

2016	Pregnancy Count/ Breed count	Pregnancy rate	2017	Pregnancy Count/ Breed count	Pregnancy rate	2018	Pregnancy Count/ Breed count	Pregnancy rate
Jan.	18/22	81.82%	Jan.	24/30	80%	Jan.	16/24	66.67%	Average before feeding (Jan.~Oct.) 71%
Feb.	26/31	83.87%	Feb.	17/34	50%	Feb.	34/40	85%
Mar.	37/45	82.22%	Mar.	37/51	72.55%	Mar.	18/23	78.26%
Apr.	12/13	92.31%	Apr.	16/21	76.19%	Apr.	26/34	76.47%
May.	25/31	80.65%	May.	34/51	66.67%	May.	19/26	73.08%
Jun.	27/36	75%	Jun.	24/31	77.42%	Jun.	13/19	68.42%
Jul.	26/31	83.87%	Jul.	15/25	60%	Jul.	30/39	76.92%
Aug.	20/31	64.52%	Aug.	20/33	60.61%	Aug.	23/40	57.50%
Sep.	24/30	80%	Sep.	21/28	75%	Sep.	16/25	64.00%
Oct.	18/21	85.71%	Oct.	27/37	72.97%	Oct.	27/39	69.23
Nov.	22/36	61.11%	Nov.	33/45	73.33%	Nov.	27/30	90%	Average after feeding 89%
Dec.	24/34	70.59%	Dec.	20/28	71.43%	Dec.	29/33	87.88%

An upper table indicates that pregnancy rates, from which averaged 78% in 2016 and 70% in 2017, and 71% in October 2018, increased until more than 87% after feeding Amisan-1000 since October 2018.

III. Weight gain after feeding Amisan-1000 for pigs during 2 months (60 days)

The test group was feed through diluting 1L of Amisan-1000 with 1 ton of water.

Table 4. weight gain table after feeding Amisan-1000

Test period	Total weight (kg)	Pigs Count	Average weight (kg)	Condition	Remarks
25 July 2017 ~ 26 Sep 2017	29.050	1,100	26.41	-before feeding
24 Oct 2017 ~ 12 Dec 2017	22.330	810	27.56	-during feeding	1.15kg weight gain
26 Dec 2017 ~ 6 Feb 2018	32.160	1,050	31.05	-after feeding	3.5kg weight gain

The upper table indicates that average weight of pigs increased more than 4.6kg in comparison to before feeding after feeding during 60 days.

Source : Test result of swine farm having 10,000 pigs count

IV. Weight gain of 4-week-old weaning piglets in case of being fed Amisan-1000

Table 5. Weight gain example of 4-week-old weaning piglets in case of being fed Amisan-1000 [The Data in 2019]

Date	Swine Count	Total weight (kg)	Average weight (kg)	Remarks
14 Mar. 21 Mar. 28 Mar. 04 Apr. 11 Apr. 18 Apr. 25 Apr. 02 May 09 May 16 May 25 May	207 209 198 210 235 212 260 245 240 190 242	2005.8kg 2092.8kg 1817.6kg 2129.4kg 2305.4kg 1975.8kg 2399.8kg 2205.0kg 2354.4kg 1710kg 2524kg	9.69kg 10.11kg 9.18kg 10.14kg 9.81kg 9.32kg 9.23kg 9.00kg 9.81kg 9.00kg 10.43kg

* Average weight of 4-week-old weaning piglet in South Korea is 5.5kg~6.5kg

V. Weight gain of 7-week-old weaning piglets in case of being fed Amisan-1000

Table 6. Weight gain example of 7-week-old weaning piglets in case of being fed Amisan-1000 [The Data in 2019]

Date	Swine Count	Total weight (kg)	Average weight (kg)	Remarks
28 Mar. 04 Apr. 11 Apr. 18 Apr. 23 May.	204 198 210 205 240	3616.9kg 3752.1kg 3853.5kg 3599.8kg 4699.0kg	17.73kg 18.95kg 18.35kg 17.56kg 19.58kg

VI. 10-week-old (70-day-old) weaning piglets in case of being fed Amisan-1000

Table 7. Weight gain example of 10-week-old (70-day-old) weaning piglets in case of being fed Amisan-1000 [The Data in 2019]

Date	Swine Count	Total weight (kg)	Average weight (kg)	Remarks
4 Apr. 11 Apr. 18 May	204 198 204	6721.8kg 7078.5kg 6940.0kg	32.95kg 35.75kg 34.02kg

* Average weight of 10-week-old (70-day-old) weaning piglet is 26kg in South Korea : 26kg

VII. shipment date in case of being fed Amisan-1000 during 60 days after weaning

- First shipment age : 150 days
- Average shipment age : 160 ~ 170 days

* Average shipment age in South Korea : 202 days

VIII. Effects on Productivity and Immunity Enhancement in broiler in case of feeding of Amisan-1000

12,000 male chick counts of 1 day-old broiler (Ross x Ross 308) were selected, and that the test conducted for 32 days at the beginning of the stage of broiler (0~3 weeks) and the end of the stage of broiler (3~5 weeks). The test field place is a broiler farm in Jeong-Eup City in South Korea.
A test group and a control group were seperated and bred in two general cage-free hen houses with 6000 counts of chick respectively. A forage was Cheonha-Jaeil-Feed, which was fed at the beginning of the breeding stage of broiler and the end of the breeding stage of broiler. Both the test group and the control group were given free feeding. The test group was fed through diluting 1L of Amisan-1000 per 1 ton water, and the control group drank general water freely.

Table 8. Effects of Amisan-1000 on growth rate, feed intake, feed conversion rate in broiler

Items	Control group	Amisan-1000 group
Initial BW (g) Final BW (g) BW gain (g) Feed intake (g) FCR	42 1,612 1,570 2,744 1.61	42 1,665 1,623 2,789 1.60

Table 9. Effects of Amisan-1000 on leukocyte, erythrocyte, lymphocyte, Ig G in broiler

Items	Control group	Amisan-1000 group
WBC, 103/ul RBC, 106/ul Lymphocyte, % IgG, mg/dL	288.6 2.12 82.3 1.27	297.3 2.19 82.5 1.35

In broiler experiments, the weight gain indicated a tendency to increase in the test group of being fed Amisan-1000(1,665g), compared to the control group (1,612g).
And, the feed intake indicated a tendency to increase in the Amisan-1000 group (2,798g) in comparison to the control group (2,744g).
Also, Feed Conversion Rate (FCR) has a significantly improved a little in Amisan-1000 group (1.61) in comparison to the control group (1.60).
The effects on blood properties also showed some improvement in leukocyte, erythrocyte, lymphocyte, Ig G content.

Source : Analysis result report on productivities and immunity enhancement effects assessment of Amisan-1000 in piglets and broilers. (by INVIVO Research Company, 2017)